CO PROCHECK Vitamin D Rapid Test Cassette

The Vitamin D Rapid Test Cassette (Fingerstick Whole Blood) test mixture migrates upwards on the strip by capillary action. The membrane is precoated with 25(OH)D antigens on the test line region of the strip. During testing, 25(OH)D present in the specimen will compete with 25(OH)D on the test line. The higher concentration of 25(OH)D in the specimen, the lighter would be the T line. The result will be read according to the colour card provided with the kit. To serve as a procedural control, a coloured line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred. • Wash your hands with soap and rinse with clear warm water. • Bring the pouch to room temperature before opening it. Open the pouch, remove the test cassette and place it on a clean and level surface. • Run the test within one hour and best results will be obtained if the test is performed immediately after opening the foil pouch. • Remove the dropper, buffer vial, lancet and alcohol pad, and place them close to the test cassette. 1. Carefully pull off and dispose the released cap of the lancet. 2. Use the provided Alcohol pad to clean the fingertip of the middle or ring finger as the puncture site. Allow to air dry. 3. Press the lancet, on the side from where the cap was extracted; the tip retracts automatically and safely after use. Massage the hand without touching the puncture site by massaging the hand towards the fingertip of the middle or ring finger to be punctured. 4. Keeping the hand down and massage the end that was pricked to obtain a blood drop. 5. Without squeezing the capillary dropper bulb, put it in contact with the blood. The blood migrates into the capillary dropper through the capillarity to the line indicated on the capillary dropper. You may massage again your finger to obtain more blood if the blood does not reach the indicated line. Ensure no air bubbles. 6. Release the blood collected into the Specimen well (S) of the cassette, by squeezing the dropper bulb. 7. Wait for the blood to be totally dispensed in the well. Unscrew the cap of the buffer bottle and add 2 drops of buffer into the Buffer well (B) of the cassette and start a timer. 8. Wait for the coloured line(s) to appear. Read results at 10 minutes. Compare the T line intensity with the “Vitamin D Colour card” provided with the kit to get the Vitamin D level in your blood. Do not interpret the result after 20 minutes, as results will be unreliable.

• For self-testing in vitro diagnostic use only. • Store in a dry place at 2-30°C, avoiding areas of excess moisture. If the foil packaging is damaged or has been opened, please do not use. • This test kit is intended to be used as a preliminary test only and repeatedly abnormal results (i.e. deficient, insufficient and excess) should be discussed with a doctor or medical professional. • Follow the indicated time strictly. • Use the test only once. Do not dismantle and touch the test window of the test cassette. • The kit must not be frozen or used after the expiration date printed on the package. • Keep out of the reach of children. • The used test should be discarded according to local regulations. • Children under the age of 14 should finish the test with the help of an adult. Always read the label and follow the directions for use.

For detection of 25-hydroxyvitamin D- 25(OH)D which is the main storage form of vitamin D in the body. Therefore, the overall presence of vitamin D can be determined by detecting the content of 25(OH)D. 25(OH)D levels less than 30 ng/mL may indicate Vitamin D Deficiency or Insufficiency. Vitamin D supplements may be recommended in these cases of vitamin D deficiency and monitoring of effectiveness of supplements can be tested for with this test.